"This is the moment to chuck Chequers", Johnson said to applause and shouts of "bravo" at the Conservative Party conference in the English city of Birmingham on Tuesday.
He also criticised Jacob Rees-Mogg for pledging financial security in Britain after Brexit at the same time as opening an investment fund in Ireland to transfer his money over.
In her first Conservative conference speech as leader in 2016 she railed against global elites, while her election manifesto rejected "untrammelled free markets".
Just six months before Britain leaves on March 29, negotiations with the European Union have ground to a halt and May is caught between Brexiteers like Johnson and those who want to keep close economic ties with the bloc, Britain's biggest trading partner.
However, Toyota became the latest high-profile business to warn that leaving the world's biggest trading bloc without any trading agreement could add costs and cripple output at plants which rely on the just-in-time delivery of tens of thousands of components. May's policies. He even posted a picture of himself online running through a wheat field, an obvious attempt to mock Ms.
She argues that her plan is the only way to avoid customs checks along the now invisible border between the U.K.'s Northern Ireland and European Union member Ireland - a development that would be troublesome for residents and businesses on both sides, and could undermine Northern Ireland's peace process.
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Hoffenheim had to contend with a host of injuries for its first game at home in Europe's Premier Competition. Hoffenheim drew 2-2 with Shakhtar Donetsk in Ukraine. "I'm not surprised", he added.
Mr Johnson's visit to the party conference is a one-day event amid mounting criticism of his recent actions in speaking out against the government.
Ian Livermore, 65, from south Gloucestershire said the speech was "superb" and showed why Johnson should be leader.
"If we all go off in different directions in pursuit of our own visions of the flawless Brexit, we risk ending up with no Brexit at all", she said. May's Brexit strategy, saying she's a "captive of the establishment", but he said a tumultuous leadership fight now would be "a disaster" for the party. "That's what we've all been saying throughout this conference", Steve Baker, who recently resigned from Ms.
Johnson, who left the government during the summer over his opposition to May's Brexit plans and is the bookmakers' favorite to replace her, has declined to say whether he would challenge her for the leadership. "We need to support the Prime Minister, but we're out to change the policy".
Michael Edwards from Hastings, a Tory member for 40 years, said Johnson gave an "inspiring speech".
He said he was anxious the United Kingdom was "so demoralised and so exhausted" that it might submit to foreign rule, a clear attack on May's Brexit plans.
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That's all for the existing family of Surface devices, but a brand new addition to the family isn't completely off the cards. Hopefully, however, this is all prelude to some price cuts that bring the Surface Laptop down to a price worth paying for.
When asked before the fringe event how she had been getting on with Mr Johnson, the prime minister laughed and told BBC One's Breakfast: "Well, I'm sure that's going to be a very lively event".
"I think he really set the right tone, I was anxious he would be divisive and speak against the Prime Minister".
"He is absolutely right about the threat that Chequers poses to our democracy, our country and ultimately the fortunes of the Conservative Party if we stick with it", Andrew Bridgen, a Conservative lawmaker, said.
"But we have been very clear, we have got to deliver a deal for the United Kingdom that's good for our economy, make sure people continue to have the opportunity to have job security and job opportunities for the future and respect that referendum and deliver on getting back control of our border, of our laws, of our money and that's what this package does".
Mr Johnson has branded the Prime Minister's Chequers plan "deranged" and compared it to putting a "suicide vest" on the British constitution.
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These teams met in the Carabao Cup on Wednesday, where the Blues defeated the Reds 2-1 at Anfield. I think we can fight for the Champions League places.